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. 2020 Jun 19;11:1254. doi: 10.3389/fimmu.2020.01254

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Copyright © 2020 Lisk, Yuen, Kuniholm, Antos, Reiser and Wetzler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Adjuvants effect colocalization of antigen onto FDCs. (A) Representative IHC images from draining lymph nodes from B6 control mice 24 h post subcutaneous injections. FDCs are shown in green. OVA-594, used as a non-immunogenic antigen, is shown in red. Areas of colocalization are shown with yellow arrows. Scale bar represents 20 μM. One out of 3 representative experiments is shown. Images were taken at 63x objective using a Leica SP5 microscope. (B) Quantification of Colocalization of fluorescently labeled OVA-A594 with FDCs within draining lymph nodes 24 h post subcutaneous injections. Colocalization was assessed using Pearson Correlation coefficients calculated with JaCoP plugin in ImageJ after background subtraction. n = 9–12, **p < 0.01, ****p < 0.001.