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. Author manuscript; available in PMC: 2020 Jun 26.
Published in final edited form as: J Vis Exp. 2019 Oct 24;(152):10.3791/60346. doi: 10.3791/60346

Table 2: Recommended cell numbers for organoid xenografting experiments.

Columns from left to right include absolute total cell number for 10 injections, total volume of PBS + Y-27632 for 10 injections, basement membrane matrix volume, and cell number per injection.

Total Cell number PBS0+ Y-27632 Matrigel volume cell concentration / Injection
5 × 106 500 μl 500 μl 5 × 105
10 × 106 500 μl 500 μl 1 × 106
15 × 106 500 μl 500 μl 1.5 × 106
20 × 106 500 μl 500 μl 2 × 106