Figure 4.

Effects of SYT4 overexpression on the protein and mRNA expression levels of melanogenesis‐related genes in alpaca melanocytes. A, Results from the qPCR analyses of SYT4, MITF, and DCT mRNA expression in melanocytes with SYT4 overexpressed or knocked down compared with the expression in the cells of the NC groups. B, Results from the Western blot and abundance analyses of SYT4, MITF, TYR, TYRP1, and DCT protein expression in melanocytes with SYT4 overexpressed or knocked down compared with the expression in the cells of the NC group. C, Results from the qPCR analyses of SYT4, MITF, TYR, TYRP1 and DCT mRNA expression in melanoma B16‐F10 cells with SYT4 overexpressed and knocked down compared with the expression in the cells of the NC groups. D, Western blot and abundance analyses of SYT4, MITF, TYR, TYRP1 and DCT protein expression in B16‐F10 melanoma cells with SYT4 overexpressed or knocked down compared with expression in the cells of the NC groups. Data represent mean ± standard error (n = 3). ^** P < .05. ^*** P < .001