Table 1.
Primers used in this study
| Primer Name | Primer Sequence 5′‐3′ | Annealing Temperature, °C |
|---|---|---|
| SYT4‐F | ATAGCGGCCGCATGGCTCCTATCACCACCAGCCGC | 64 |
| SYT4‐R | ATCCTCGAGTATGGCTCCTATCACCACCAG | |
| SYT4‐F‐RT | CCTCGTCTTCACTGTCTCTCTCTTT | 58 |
| SYT4‐R‐RT | TTCACTTCACTCTTGTCATCTCCTC | |
| MITF‐F (alpaca) | TCCCAAGTCAAATGATCCAG | 52 |
| MITF‐R (alpaca) | GAGCCTGCATTTCAAGTTCC | |
| DCT‐F (alpaca) | CCAGGGCGCCCATACAAGGC | 60 |
| DCT‐R (alpaca) | GCTGGTCTGTGCACACN(1/2G,1/2A)TCA | |
| 18S‐F (alpaca) | GAAGGGCACCACCAGGAGT | 50‐64 |
| 18S‐R (alpaca) | CAGACAAATCACTCCACCAA | |
| MITF‐F (mus) | GAGCAGAGCAGGGCAGAGAGTGAGT | 60 |
| MITF‐R (mus) | TGGGAAGGTTGGCTGGACAGGAGTT | |
| TYR‐F (mus) | TGAAAATCCTAACTTACTCAGCCCA | 60 |
| TYR‐R (mus) | TCAAACTCAGACAAAATTCCACATC | |
| TYRP1‐F (mus) | CCATTGCTGTAGTGGCTGCGTTGTT | 61 |
| TYRP1‐R (mus) | GGAGAGGCTGGTTGGCTTCATTCTT | |
| DCT‐F (mus) | TTGCTCTTGGGGTTGCTGGCTTTTC | 61 |
| DCT‐R (mus) | TCCTCCGTGTATCTCTTGCTGCTGA | |
| β‐actin (mus) | TTGCTGACAGGATGCAGAAG | 50‐64 |
| β‐actin (mus) | ACATCTGCTGGAAGGTGGAC |