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. 2019 Oct 10;98(4):408–417. doi: 10.1111/aos.14272

Figure 1.

Figure 1

Molecular analysis of Cx 36 and Cx 45 transcript and protein in retina and ON homogenates. (A) representative Western blot of homogenates prepared from rat retina and proximal and distal optic nerve stumps to detect the presence of Cx36 and Cx45. For the WB analysis, 2 retinas or 2 optic nerve stumps were pooled to obtain proper protein content. ON = optic nerve. (B) representative PCR of homogenates prepared from rat retina and proximal and distal optic nerve stumps to detect the presence of Cx36 and Cx45 transcripts. For the PCR analysis, 2 retinas or 2 optic nerve stumps were pooled to obtain proper RNA concentration. Rat brain cortex was used as a positive control and RNA‐free water as a negative control. ON = optic nerve.