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. 2020 Mar 23;47(7):1182–1192. doi: 10.1111/1440-1681.13271

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© 2020 The Authors. Clinical and Experimental Pharmacology and Physiology published by John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Involvement of the NRF‐2/DDAH1/ADMA pathway in TGF‐induced EndMT in human umbilical vein endothelial cells (HUVECs). HUVECs were treated with medium (Control), TGF‐β1, TGF‐β1 + Vector, TGF‐β1 + oeNRF‐2 and TGF‐β1 + oeNRF‐2 + siDDAH1. A, B, The mRNA expression of NRF‐2 and DDAH1 was detected. C–G, The levels of NO (C), NOS (D), IL‐1β (E), TNF‐α (F) and ADMA (G) were detected. H, The protein levels of VE‐cadherin, vimentin, DDAH, PRMT and NRF‐2 were detected. I, J, Immunofluorescence detection of CD31 (I) and α‐SMA (J) at a magnification of 200 ×. *P < .05, **P < .01, ***P < .001 vs Control, siNC or Vector; ^# P < .05, ^## P < .01, and ^### P < .001 vs TGF + Vector; ⁺ P < .05, ⁺⁺ P < .01, and ⁺⁺⁺ P < .001 vs TGF + oeNRF‐2. (H) , Control; , TGF‐β1; , TGF‐β1 + Vector; , TGF‐β1 + oeNRF2; , TGF‐β1 + oeNRF2 + siDDAH1

Inline graphic — Involvement of the NRF‐2/DDAH1/ADMA pathway in TGF‐induced EndMT in human umbilical vein endothelial cells (HUVECs). HUVECs were treated with medium (Control), TGF‐β1, TGF‐β1 + Vector, TGF‐β1 + oeNRF‐2 and TGF‐β1 + oeNRF‐2 + siDDAH1. A, B, The mRNA expression of NRF‐2 and DDAH1 was detected. C–G, The levels of NO (C), NOS (D), IL‐1β (E), TNF‐α (F) and ADMA (G) were detected. H, The protein levels of VE‐cadherin, vimentin, DDAH, PRMT and NRF‐2 were detected. I, J, Immunofluorescence detection of CD31 (I) and α‐SMA (J) at a magnification of 200 ×. *P < .05, **P < .01, ***P < .001 vs Control, siNC or Vector; ^# P < .05, ^## P < .01, and ^### P < .001 vs TGF + Vector; ⁺ P < .05, ⁺⁺ P < .01, and ⁺⁺⁺ P < .001 vs TGF + oeNRF‐2. (H) , Control; , TGF‐β1; , TGF‐β1 + Vector; , TGF‐β1 + oeNRF2; , TGF‐β1 + oeNRF2 + siDDAH1