Figure 3.

The KinCon biosensor system. Schematic depiction of the cell‐based KinCon reporter principle. A hypothetical kinase consisting of C lobe, N lobe, and AIM is indicated and fused to fragments 1 and 2 (F[1]/F[2]) of a luciferase PCA. We propose that the opened full‐length kinase conformation reflects the active one for many kinases (= ON state). The closed full‐length kinase conformation is the auto‐inhibited and inactive state (= OFF state) through the actions of an auto‐inhibitory module (AIM). Indicated means affect the opened, intermediate, or closed full‐length kinase conformations and may lead to an increase or decrease of luciferase‐PCA emitted cellular bioluminescence. PCA, protein‐fragment complementation assay