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. Author manuscript; available in PMC: 2021 Jan 21.
Published in final edited form as: Anal Chem. 2019 Dec 31;92(2):2229–2236. doi: 10.1021/acs.analchem.9b04950

Figure 2.

Figure 2.

(a) Representative image of TAMRA-labeled Nanodiscs (left) and imaging buffer (right). Samples were excited using 561 nm laser with 9.14 mW power and an exposure time of 60 ms. (b) Time-stamped montage of TAMRA-labeled Nanodiscs. (c) Representative background-subtracted trajectories of TAMRA-labeled Nanodiscs showing single (top) and double-step (bottom) photobleaching. (d) The intensity distribution of individual TAMRA-labeled Nanodiscs (N = 250). (e) Distribution of bleaching steps in the individual traces. Percentage of occurrence based on single, double and multiple bleaching step events (N = 1374). Scale bar: 5 μm.