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. 2020 Jun 26;21:47. doi: 10.1186/s12860-020-00288-9

Table 1.

Detailed specification of primary and secondary antibodies employed for western blotting

Primary antibody Supplier Catalog number Dilution Secondary antibody Supplier Catalog number Dilution
Annexin A1 LifeSpan Biosciences LS-C382041 (polyclonal) 1:500 IRDye 800CW Goat LI-COR Biosciences 926–32,211 1:10,000
Anti-Rabbit IgG
LRP1 Abcam ab92544 (monoclonal) 1:400 IRDye 680RD Goat LI-COR Biosciences 926–68,070 1:8000
Anti-Mouse IgG
MMP-1 (C-terminal) Aviva Systems Biology ARP42040 (polyclonal) 1:1000 IRDye 800CW Goat LI-COR Biosciences 926–32,211 1:10,000
Anti-Rabbit IgG
MMP-13 (middle region) Aviva Systems Biology ARP56350 (polyclonal) 1:500 IRDye 800CW Goat LI-COR Biosciences 926–32,211 1:10,000
Anti-Rabbit IgG
MMP-3 (middle region) Aviva Systems Biology ARP42042 (polyclonal) 1:1000 IRDye 800CW Goat LI-COR Biosciences 926–32,211 1:10,000
Anti-Rabbit IgG
TSP-1 Abcam ab1823 (monoclonal) 1:500a; IRDye 680RD Goat LI-COR Biosciences 926–68,070a; 1:5000a
1:1000b Anti-Mouse IgGa;
IRDye 800CW Goat 926–32,210b 1:10,000b
Anti-Mouse IgGb
VDAC1 (C-terminal) Aviva Systems Biology ARP35122 (polyclonal) 1:250 IRDye 680RD Goat LI-COR Biosciences 926–68,071 1:5000
Anti-Rabbit IgG
VDAC2 (N-terminal) Aviva Systems Biology ARP35123 (polyclonal) 1:250 IRDye 680RD Goat LI-COR Biosciences 926–68,071 1:5000
Anti-Rabbit IgG
β-actin LI-COR Biosciences 926–42,210 (monoclonal) 1:500 IRDye 800CW Goat LI-COR Biosciences 926–32,211 1:10,000
Anti-Rabbit IgG

aEmployed on total cell lysates. bEmployed on conditioned media samples