Table 1.
Primary antibody | Supplier | Catalog number | Dilution | Secondary antibody | Supplier | Catalog number | Dilution |
---|---|---|---|---|---|---|---|
Annexin A1 | LifeSpan Biosciences | LS-C382041 (polyclonal) | 1:500 | IRDye 800CW Goat | LI-COR Biosciences | 926–32,211 | 1:10,000 |
Anti-Rabbit IgG | |||||||
LRP1 | Abcam | ab92544 (monoclonal) | 1:400 | IRDye 680RD Goat | LI-COR Biosciences | 926–68,070 | 1:8000 |
Anti-Mouse IgG | |||||||
MMP-1 (C-terminal) | Aviva Systems Biology | ARP42040 (polyclonal) | 1:1000 | IRDye 800CW Goat | LI-COR Biosciences | 926–32,211 | 1:10,000 |
Anti-Rabbit IgG | |||||||
MMP-13 (middle region) | Aviva Systems Biology | ARP56350 (polyclonal) | 1:500 | IRDye 800CW Goat | LI-COR Biosciences | 926–32,211 | 1:10,000 |
Anti-Rabbit IgG | |||||||
MMP-3 (middle region) | Aviva Systems Biology | ARP42042 (polyclonal) | 1:1000 | IRDye 800CW Goat | LI-COR Biosciences | 926–32,211 | 1:10,000 |
Anti-Rabbit IgG | |||||||
TSP-1 | Abcam | ab1823 (monoclonal) | 1:500a; | IRDye 680RD Goat | LI-COR Biosciences | 926–68,070a; | 1:5000a |
1:1000b | Anti-Mouse IgGa; | ||||||
IRDye 800CW Goat | 926–32,210b | 1:10,000b | |||||
Anti-Mouse IgGb | |||||||
VDAC1 (C-terminal) | Aviva Systems Biology | ARP35122 (polyclonal) | 1:250 | IRDye 680RD Goat | LI-COR Biosciences | 926–68,071 | 1:5000 |
Anti-Rabbit IgG | |||||||
VDAC2 (N-terminal) | Aviva Systems Biology | ARP35123 (polyclonal) | 1:250 | IRDye 680RD Goat | LI-COR Biosciences | 926–68,071 | 1:5000 |
Anti-Rabbit IgG | |||||||
β-actin | LI-COR Biosciences | 926–42,210 (monoclonal) | 1:500 | IRDye 800CW Goat | LI-COR Biosciences | 926–32,211 | 1:10,000 |
Anti-Rabbit IgG |
aEmployed on total cell lysates. bEmployed on conditioned media samples