Table 2.

MSDIN family members searched from the transcriptomes of seven amanitin-producing mushrooms

Name	No.	Leader peptide	Core peptide	Recognition sequence	Product
A. exitialis	Ae1	MTDINDTRLP	FIWLLWIWLP	SVGDDNNILNRGEDLC*
	Ae2	MSDINATRLP	LFFPPDFRPP	CVGDADNFTLTRGENLC*
	Ae3	MSDVNATRLP	FNFFRFPYP	CIGDDSGSALRLGESLC*
	Ae4	MSDINTARLP	IPVPPFFIP	FVGDDIDVVLRRGENLC*
	Ae5	MSDINVTRLP	VFIFFFIPP	CVGDGTADIVRKGENLC*
	Ae6	MSDINTARLP	VFSLPVFFP	FVSDDIQAVLTRGESLC*
	Ae7	MSDINTTRLP	FVFVASPP	CVGDDIAMVLTRGENLC*
	Ae8	MSDINPTRLP	IFWFIYFP	CVSDVDSTLTLCISLS*
	Ae9	MSDINTARLP	IIWIIGNP	CVSDDVERILTRGESLC*
	Ae10	MSDINATRLP	IIWAPVVP	CISDDNDSTLTRGQSLC*
	Ae11	MSDINATRLP	IGRPQLLP	CVGGDVNYILISGENLC*
	Ae12	MSDINATRLP	IWGIGCDP	CVGDDVTALLTRGEALC*	β-amanitin
	Ae13	MSDINATRLP	IWGIGCNP	CVGDDVTSVLTRGEALC*	α-amanitin
	Ae14	MSDINVIRLP	SMLTILPP	CVSDDASNTLTRGENLC*
	Ae15	MSDINATRLP	AWLTDCP	CVGDDVNRLLTRGESLC*	“phallotoxin”
	Ae16	MSDINATRLP	AWLVDCP	CVGDDVNRLLTRGESLC*	phallacidin
	Ae17	MSDINLTRLP	GIIAIIP	CVGDDVNSTLTRGQSLC*
	Ae18	MSDINATRLP	VWIGYSP	CVGDDCIALLTRGEGLC*
	Ae19	MSDINATRLP	GFLFWA	YVGDDVDYILTRGESLA*
A. fuliginea	Af1	MSDINATRLP	IIIVLGLIIP	LCVSDIEMILTRGESLC*
	Af2	MSDLNASRLP	ILSVLGLPVP	HVGEETNSTLARGESLC*
	Af3	MSDINSARLP	LFFPPIFIPP	CVSDDVQVVLTRGENLC*
	Af4	MSDINAARLP	FFPFVFIPP	CIGDDATSIVRQAENLC*
	Af5	MSDINTIRIP	FPWTGPFVP	CVSDDVGSVLMRGESLS*
	Af6	MSDTNATRLP	IWFIQLQIP	CAGDDVNSSLTRGESLC*
	Af7	MSDINVTRLP	VLVFIFFPP	YISDDAVNILKQGENLC*
	Af8	MFDINGSRLP	AFRLIPPP	CVGDDVDSTLTSGESLC*
	Af9	MSDINATRLP	GILIVFPP	CVGDDVNSTLTRGESLC*
	Af10	MSDINATRLP	HLFTWIPP	CISDDSTLTRGESFC*
	Af11	MFDINSSRLP	HLYPNSRP	CVCDDACSTLTSAESLC*
	Af12	MSDINATRLP	IFWFIYFP	CVGDDVDNTLTRGESLS*
	Af13	MSDINATRLP	IWGIGCDP	CVGDDVAALITRGEALC*	β-amanitin
	Af14	MSCINATRLP	LPSRPVFP	FVSDAIEVVLGRGEDLC*
	Af15	MSDINSLRLP	VVNSRFNP	CVGDDVSPTLTRGEGLC*
	Af16	MSDINASRLP	AWLATCP	CIGDDVNPTITRGESLC*	phalloidin
	Af17	MSDINATRLP	AWLVDCP	CVGDDVNRLLARGENLC*	phallacidin
	Af18	MSDINATRLP	AWLVTCP	CVGDDINRLLTRGENLC*	“phallotoxin”
A. molliuscula	Am1	MSDINTARLP	YFLPPIFSPP	CVSDDIEMVLTRGENLC*
	Am2	MTDINATRLP	ILFGFFLLP	CVDGVDNTLHSGENLC*
	Am3	MSNINASRLP	IWAAFFRFP	CVGDEVDGILRSGESLC*
	Am4	MSDINATRLA	IWGIGCDP	CVGDDVTALLTRGEALC*	β-amanitin
	Am5	MSDINASRLP	RLLVPRYP	CIDEDAEGATYLC*
	Am6	MSNINAIRLP	GFFAVVP	YLATSITFSLLGRGESLC*
	Am7	MTDINATRLP	WIFFFPP	CVDDVDNTLHSGENLC*
	Am8	MSNINALRLP	GFGFIP	YASGDVDYTLTRGESLS*
	Am9	MSDINATRFP	GKVNPP	YVGDDVDDIIIRGEKLC*
A. pallidorosea	Ap1	MADINAARLP	FHGLFPFLPPP	FVDDDATSTLTRGESLC*
	Ap2	MADINASRLP	LNILPFHLPP	CVSDDATSTLTRGESLC*
	Ap3	MSDINATRLP	NWHAGPTRPP	CVADDVSLTLTRGESLC*
	Ap4	MSDINTARLP	VFFMPPFIPP	CVSDDIQMVLTRGENLC*
	Ap5	MSDINTARLP	EFIVFGIFP	CVGDDIQTVLTRGEDLC*
	Ap6	MSDINASRLP	FFPEVGFFP	CVGDDTNPILTRGGSLS*
	Ap7	MSDLNATRLP	FNLFRFPYP	CIGDDSGSVLTLGEGLC*
	Ap8	MSDINTIRVP	FPWTGPFVP	CVGDDVGSVLTHGESLS*
	Ap9	MSDINATRLP	HPFPLGLQP	CAGDVDNLTLFRGEGLC*
	Ap10	MSDINATRLP	DPRRLLIP	GSSDDVDSALTRGESLC*
	Ap11	MSDINTTRLP	HFFNLMPP	CVGDDIETVLTRGESLC*
	Ap12	MSDINATRLP	HQHHPFVP	GGSDDVGSTLTRGESLC*
	Ap13	MSDMNVVRLP	ISDPTAYP	CVGDDIQAVLGRGESLC*
	Ap14	MSDINATRLP	IWGIGCDP	CVGDDVTAVLTRGEALC*	β-amanitin
	Ap15	MSDINATRLP	IWGIGCNP	CVGDEVAALLTRGEALC*	α-amanitin
	Ap16	MSDINATRLP	IWGIGCNP	CVGDEVTALITRGEALC*	α-amanitin
	Ap17	MSDINATRLP	LGRPESLP	CVGDDVNYILVSGGNLS*
	Ap18	MSDINAARLP	LVYMILFP	SVGDDIDVVLGRGENLC*
	Ap19	MSDVNATRLP	MAFPEFLA	CVGDDVNHTLTRGERLC*
	Ap20	MSDINTARLP	MHILAPPP	CVSDDIEMVLTRGESLC*
	Ap21	MSDINAARLP	NLFVWIPP	CISDDINSTLTRGESLC*
	Ap22	MSDINTTRLP	YMWDHHLP	CASDDIQMVFTRGENLC*
	Ap23	MSDINASRLP	AWLATCP	CAGDDVNPTLTRGESLC*	phalloidin
	Ap24	MSDINATRLP	AWLMTCP	CVGDDVNPTLTRGESLC*	“phallotoxin”
	Ap25	MSDVNATRLP	AWLVDCP	CVGDDINRLLTRGENLC*	phallacidin
A. rimosa	Ar1	MSDINTSRLP	FIPLGIITILP	CVSDDVNTTITRGESLC*
	Ar2	MTDINDTRLP	FVWILWLWLA	CVGDDTSILNRGEDLC*
	Ar3	MSDINATRLP	IIIVLGLIIP	LCVSDIEMILTRGESLC*
	Ar4	MSDVNTTRLP	FNFFRFPYP	CICDDSEKVLELGENLC*
	Ar5	MSDINATRLP	HPFPLGLQP	CAGDVDNFTVSCHSLC*
	Ar6	MLDINATRFP	LGRPTHLP	CVGDDVNYILIGNGENLC*
	Ar7	MSDINASCLP	LILVANGMA	YVSDDVSPTLTRGENLC*
	Ar8	MPDINVTRLP	LLIIVLLTP	CISDDNNILNRGKDLC*
	Ar9	MSDIHAARLP	FPTRPVFP	SAGDDMIEVVLGRGEDLC*
	Ar10	MSDNNAARLP	FYFYLGIP	SDDAHPILTRGESLC*
	Ar11	MSDINIARLP	IFWFIYFP	CVGDDVDNTLSRGESLS*
	Ar12	MSDINASRLP	ILKKPWAP	SVCDDVNSTLTRGEGLC*
	Ar13	MSDINVARLP	ISDPTAYP	CVGDDIQAVVKRGESLC*
	Ar14	MSDINATRLP	IWGIGCDP	CVGDDVAALTTRGEALC*	β-amanitin
	Ar15	MSDINSTRLP	IWGIGCNP	SVGDEVTALLTRGEALC*	α-amanitin
	Ar16	MSDINATRLP	AWDSKHP	CVGDDVSRLLTRGESLC*
	Ar17	MSDINATRVP	AWLAECP	CVGDDISHLLTRGENLC*	“phallotoxin”
	Ar18	MSDINATRVP	AWLVDCP	CVGDDISRLLTRGENLC*	phallacidin
A. subpallidorosea	Asp1	MTDVNDTRLP	FIWLIWLWLP	SVGDDINILNGGEDLC*
	Asp2	MTDINYARLP	ITLFLFFFIP	CLSDDDNILNRGKDLC*
	Asp3	MSDINTARLP	YFLPPIFSPP	CVSDDIEMVLTRGENLC*
	Asp4	MSDINATRLP	HPFPLGLQP	CAGDVDNFTLTKGEDLC*
	Asp5	MSDINATRLP	GILIVWPP	CVGDDVNFTLTRGESLC*
	Asp6	MSDINTTRLP	IAFPEFIA	RVGDDIHRTLTRGESLC*
	Asp7	MSDINVTRLP	IFWFIYFP	CVGDDVDNTLTRGESLS*
	Asp8	MSDINAIRLP	IGRPENKP	CVGGDVNYILISGEKLC*
	Asp9	MSDINATRLP	IVFLEFYS	CVGDDVNSTLTRGESLC*
	Asp10	MSDINATRLP	IWGIGCDP	CVGDDVAAFLTRGEALC*	β-amanitin
	Asp11	MSDINATRLP	IWGIGCNP	SVGDEVTALLTRGEALC*	α-amanitin
	Asp12	MSDINASRLP	VIGLFGLP	YVSDDVQPILTRGDSLC*
	Asp13	MSDINASRLP	VIPFLLPP	CVSDDVNFTLTRGESLC*
	Asp14	MSDINATRLP	YFRPAPPP	CVSDDINPILTCGESLC*
	Asp15	MSDINAARLP	AWITDCP	CVGDDINRILTRGENIC*	“phallotoxin”
	Asp16	MSDINASRFP	AWLATCP	CVGDDVNPTIARGESLC*	phalloidin
	Asp17	MSDINATRLP	AWLVTCP	CVGDDVNFTLTRGESLC*	“phallotoxin”
	Asp18	MSDINATRLP	AWLVTCP	CVGDDVNPTITRGESLC*	“phallotoxin”
	Asp19	MSDINTIRIP	GPFGFA	YVGDEVENLLKRGESLS*
L. venenata	Lv1	MDANATRLP	IWGIGCNP	WTPESVNDTLTKDLS*	α-amanitin
	Lv2	MDANSTRLP	IWGIGCNP	WAPESVNDTLTRGKDLC*	α-amanitin

The MSDIN members with underlined numbers were verified at the genomic level. “Phallotoxin” means a novel heptapeptide similar to the phallotoxin cyclopeptide and capable of containing tryptathione (Trp-Cys)