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. 2020 Jun 26;20:597. doi: 10.1186/s12885-020-07081-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — MiR-34c regulates the SOX2-EMT axis in NPC cell lines. a, b and c Relative expression (qRT-PCR) of SOX2: a in NP69, NP460 and, C666–1 cell lines, normalized to C666–1 cells; b in NP69-anti-miR-34c stable cells and their control; c in NP460-anti-miR-34c stable cells and their control. d and e Western blots (WBs) were performed using anti-Snail (αSnail), anti-Claudin-1 (αClaudin-1), anti-ZO-1 (αZO-1), anti-ZEB1 (αZEB1), anti-CDH1 (αCDH1), anti-CDH2 (αCDH2), with anti-β-actin (αβ-actin) as loading control. d WBs using NP69-anti-miR-34c stable cells and their control. Full-length blots are presented in Additional file 6: Figure S6. (E) WBs using NP460-anti-miR-34c stable cells and their control. ZO-1 was undetectable in NP460 cell lines. Full-length blots are presented in Additional file 7: Figure S7. f Relative SOX2 expression assessed by qRT-PCR in C666–1 cells treated with SB431542 (10 or 20 μM). The data are expressed as the mean ± SEM of at least three independent experiments. * P < 0.05; *** P < 0.001