TABLE 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Description | Source of reference |
| Strains | ||
| DH5α | F– ϕ 80dlacZΔM15 endA1 recA1 hsdR17(rK– mK+) supE44 thi-1 relA1 Δ(lacZYA-argF)U169 gyrA96 deoR | TransGen |
| S17-1 | RP4-2 Tc::Mu Km::Tn7 Tpr Smr Pro Res– Mod+ | Dr. Ramphal |
| ISP50 | Clinical isolate from sputum samples of the a female patient with acute exacerbations of chronic bronchitis before treatment with biapenem, IMPs | This study |
| IRP41 | Clinical isolate from sputum samples of the a female patient with acute exacerbations of chronic bronchitis after treatment with Biapenem for 4 days, IMPR | This study |
| Klebsiella pneumoniae ATCC 700603 | Klebsiella pneumoniae ATCC 700603 | Fuxiang Biotechnology |
| PA-NK41 | KPC2 carbapenemase producing P. aeruginosa | This study |
| PA14 | Wild type P. aeruginosa strain | Liberati et al. (2006) |
| PA14pbpC::Tn | PA14 with pbpC disrupted by insertion of Tn | Liberati et al. (2006) |
| PAO1 | Wild type P. aeruginosa strain | Kaufman et al. (2000) |
| PAO1ΔldcA | PAO1 with ldcA gene deleted | This study |
| PA14ΔldcA | PA14 with ldcA gene deleted | This study |
| ISP50ΔldcA | ISP50 with ldcA gene deleted | This study |
| PAO1ΔampR | PAO1 with ampR gene deleted | This study |
| Plasmids | ||
| pUCP24 | Shuttle vector between E. coli and P. aeruginosa; Gmr | West et al. (1994) |
| pMMB67EH-Gm | Shuttle vector between E. coli and P. aeruginosa; Gmr | Long et al. (2019) |
| pEX18Tc | Gene knockout vector, Tcr | Hoang et al. (1998) |
| pUCP24-oprDISP50 | oprD gene from ISP50 in pUCP24, Gmr | This study |
| pUCP24-oprDIRP41 | oprD gene from IRP41 in pUCP24, Gmr | This study |
| pUCP24-ampC | ampC gene from ISP50 in pUCP24, Gmr | This study |
| pUC18T-miniTn7T-ampC-His | C-terminal His-tagged ampC in pUC18T-miniTn7T, Tcr | This study |
| pUCP24-pbpC | pbpC gene from ISP50 in pUCP24, Gmr | This study |
| pUCP24-ldcAISP50 | ldcA gene from ISP50 in pUCP24, Gmr | This study |
| pUCP24-ldcAIRP41 | ldcA gene from IRP41 in pUCP24, Gmr | This study |
| pMMB-ampRISP50 | ampR gene from ISP50 in pMMB67EH-Gm, Gmr | This study |
| pMMB-ampRIRP41 | ampR gene from IRP41 in pMMB67EH-Gm, Gmr | This study |
| pEX18-ldcA | ldcA gene deletion on pEX18Tc, Tcr | This study |
| pEX18-ampR | ampR gene deletion on pEX18Tc, Tcr | This study |