Skip to main content
. 2020 Mar 13;59(20):7891–7896. doi: 10.1002/anie.201916447

Scheme 1.

Scheme 1

Synthesis of the nitroxyl spin labeled nucleoside triphosphates TPT3NO TP (1) and TPT3rNO TP (2) from TPT3I (3).9i TPT3NO TP (1): (i) propargylamine, CH2Cl2, r.t., 0.5 h, quant. (ii) CuI, NEt3, Pd(PPh3)4, DMF, r.t., o.n., 66 %. (iii) POCl3, Me3PO4, proton sponge, 0 °C, 3 h, then (Bu3NH)2PPi, NBu3, 0 °C, 30 min, 13 %. TPT3rNO TP (2): (iv) CuI, NEt3, Pd(PPh3)4, DMF, r.t., o.n., 96 %. (v) 2‐chloro‐4H‐1,2,3‐dioxaphosphirine‐4‐one, 1‐4‐dioxane, r.t., 40 min, then (Bu3NH)2PPi, NBu3, DMF, r.t., 40 min, then I2, py/H2O, 5 %.