Figure 1.

Generation of Reln‐del mice by CRISPR/Cas9. Schematic RELN gene structure in (a) human and (b) mouse. (a) The genomic region (exon 52 to 58) that was deleted in the Japanese subject with schizophrenia is shown in green.24 (b) CRISPR/Cas9 strategy for generating the model mouse with Reln deletion as shown in (a). Guide RNAs (gRNA) and single‐stranded DNA (ssDNA) for insertion of a stop codon in exon 52 of Reln gene. (c) Sequence around mouse Reln exon 52 and design of gRNA for CRISPR target and primers to perform the T7 endonuclease I (T7EI) assay for cleavage activity. (d) Results of the T7EI assay. The amount of shortened DNA shows cleavage activity by T7EI. (e) Summary of the results following injection of the CRISPR mixture into fertilized C57BL/6J egg pronucleus.