Figure 3.

Effect of 1,25(OH)₂D₃ on the production of IL‐10, TGF‐β1, IL‐17A and IL‐6 in CD4⁺ T lymphocytes depending on the presence and absence of hPDLCs. Allogenic CD4⁺ T lymphocytes were activated by 10 µg/ml PHA and co‐cultured with 100 ng/ml IFN‐γ and 100 nM 1,25(OH)₂D₃ stimulated hPDLCs for 5 days in an indirect co‐culture model. IL‐10 (a), TGF‐β1 (b), IL‐17A (c) and IL‐6 (d) levels were determined in conditioned media using ELISA. Measured cytokine concentrations were normalized to the appropriate total number of CD4‐positive T lymphocytes. All data are presented as mean ± SEM. from six independent experiments using hPDLCs isolated from six different individuals. *p‐value < .05 compared between corresponding groups as indicated