Figure 6.

Effect of different 1,25(OH)₂D₃ concentrations on PTGS‐2 expression in IFN‐γ treated hPDLCs. Primary hPDLCs were stimulated with different 1,25(OH)₂D₃ concentrations (0.01–100 nM) in the presence of 100 ng/ml IFN‐γ for 48 hr. Unstimulated and only with 100 nM 1,25(OH)₂D₃ treated cells served as control. PTGS‐2 gene expression was investigated by qPCR, showing the n‐fold expression compared to the control. GAPDH served as internal control. All data are presented as mean value ± SEM from five independent experiments with hPDLCs isolated from five different individuals. *p‐value < .05 compared to the control; #p‐value < .05 compared to IFN‐γ alone