Fig. 6.

Inhibition of ENT-mediated [³H]uridine uptake in HeLa cells by nine NRTIs. The experiments were terminated after 2 minutes. All graphs are represented by relative uptake as a percentage of control ([³H]uridine only). Three concentrations of abacavir (A), entecavir (B), zidovudine (C), didanosine (D), zalcitabine (E), emtricitabine (F), tenofovir disoproxil (G), stavudine (H), and lamivudine (I) were incubated with approximately 20 nM [³H]uridine or 20 nM [³H]uridine and 100 nM NBMPR as indicated on the x-axis. Significance between control without NBMPR and the NRTIs is indicated as *P < 0.05; ***P < 0.0001. Significance between control with 100 nM NMBPR and the NRTIs is indicated as ^†P < 0.05. Two-way analysis of variance with Bonferonni’s correction for multiple comparisons was used for statistical significance analysis using GraphPad Prism 7. Data in graphs are reported as mean ± S.D. of four replicate values (n = 3).