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. 2020 Jun 19;11:835. doi: 10.3389/fpls.2020.00835

FIGURE 3.

FIGURE 3

Local PTGS suppression activity of V2 mutants in wild-type (WT) and RDR6i N. benthamiana plants. Leaves from WT (A) and RDR6i (B) N. benthamiana plants infiltrated with a mixture of two A. tumefaciens cultures containing GFP and the desired V2 construct, were photographed under UV light at 5 dpi. Wild-type V2 protein (wt) was used as a positive control. (C) Relative GFP mRNA levels in infiltrated tissues at 5 dpi. GFP levels were normalized to EF1 and are presented as the relative amount of transcripts compared with the amount found in wild-type V2 (wt) samples (set to 100%). Bars represent mean values from a pool of 2–3 leaves obtained from 4 plants for each combination. Similar results were obtained in two independent experiments.