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. 2020 Jun 19;11:835. doi: 10.3389/fpls.2020.00835

FIGURE 4.

FIGURE 4

Expression of BCTV V2 mutants from PVX. Wild-type or mutated versions of V2 were expressed from a PVX vector and tested individually in N. benthamiana leaves. (A) Infiltrated tissue (local) at 6 dpi or apical tissue at 10 dpi (systemic) with the empty PVX vector (C) or with a recombinant PVX virus expressing the wild-type V2 protein (V2wt). (B) Infiltrated tissue (local) at 6 dpi with the empty PVX vector (C) or with a recombinant PVX virus expressing the wild-type (V2wt) or the mutated versions (P1A, H1GG, H2GG) of V2. (C,D) Measurement of the conductivity from leaf discs extracted from local (C) or systemically infected (D) tissues. Seven to eight plants were infected with each PVX-derived construct and conductivity (μS/cm) was measured in two pools of four leaf discs per plant. Bars represent mean values ± standard error (SE) for seven to eight plants (four discs per leaf were pooled and two leaves per plant were used) per recombinant PVX construct. Mean values marked with different letter (a, b, or c) indicate results significantly different from each other, as established by One Way ANOVA (Dunnett’s Multiple Comparison Test; P < 0.05). Similar results were obtained in two independent experiments.