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. 2020 Jun 19;11:835. doi: 10.3389/fpls.2020.00835

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Copyright © 2020 Luna, Romero-Rodríguez, Rosas-Díaz, Cerero, Rodríguez-Negrete, Castillo and Bejarano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Infection of N. benthamiana plants with BCTV V2 mutants. Plants were agroinoculated with wild-type or V2 mutated BCTV infective clones. Relative viral DNA accumulation in apical leaves (systemic infection) at 28 dpi (A) or at the infiltrated leaves (local infection) at 4 dpi (B). BCTV accumulation was measured by qPCR. DNA levels were normalized to 25S ribosomal DNA interspacer (ITS) and are presented as the relative amount of virus compared with the amount found in wild-type BCTV (wt) samples (in gray, set to 100%). Bars represent mean values ± standard error (SE) for 4–8 pools of two leaves from 3 to 4 plants each one. Mean values marked with different letter (a or b) indicate results significantly different from each other, as established by One Way ANOVA (Dunnett’s Multiple Comparison Test; P < 0.05).