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. 2020 Jun 19;11:835. doi: 10.3389/fpls.2020.00835

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Copyright © 2020 Luna, Romero-Rodríguez, Rosas-Díaz, Cerero, Rodríguez-Negrete, Castillo and Bejarano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Infection of A. thaliana wild-type (WT), dcl2/4 (dcl2-1/dcl4-2) and rdr6 (rdr6-15) mutants with wild-type or BCTV V2 mutants. The relative viral DNA accumulation at 28 dpi was quantified by qPCR in WT, rdr6 and dcl2/4 backgrounds. DNA levels were normalized to actin gene and are presented as the relative amount of virus compared with the amount found in wild-type BCTV (wt) samples (in gray, set to 100%). Bars represent mean values ± standard error (SE) for eight to twelve plants per biological replicate. Data from three biological replicate are shown. Mean values marked with different letter (a or b) indicate results significantly different from each other, as established by One Way ANOVA (Dunnett’s Multiple Comparison Test; P < 0.05).