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. Author manuscript; available in PMC: 2021 Apr 25.
Published in final edited form as: Methods Enzymol. 2020 Apr 25;639:167–189. doi: 10.1016/bs.mie.2020.04.013

Fig. 2.

Fig. 2.

Genetically encode Qui into EGFP chromophore to generate EGFP-66Qui. (A) Chromophore structures of EGFP and EGFP-66Qui. (B) UV–vis absorbance spectra of EGFP and EGFP-66Qui in phosphate buffer (pH 7.4). (C) Fluorescence emission spectra of EGFP-66Qui in buffers with indicated pH values. (D) pH profile of EGFP-66Qui. Fluorescence emission intensity at 505 nm of EGFP-66Qui (measured in C) was plotted against pH. Adapted with permission from Fu, C. et al. (2018). Genetically Encoding Quinoline Reverses Chromophore Charge and Enables Fluorescent Protein Brightening in Acidic Vesicles. Journal of the American Chemical Society, 140, 11058–11066. Copyright (2018) American Chemical Society.