Table 2.
Quantification of c-fos expression to examine the effect of localized yohimbine infusion on LC activity in 5 mice. Yohimbine was infused in the left LC (Mouse 1 and 2: anesthesia; Mouse 3–5: awake). The right LC serves as a basal level control. Permutation test was performed (105 iterations) to compare c-fos expression levels between the left and right LC in individual mice.
| Yohimbine | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mouse number | 1 | 2 | 3 | 4 | 5 | |||||
| Left | Right | Left | Right | Left | Right | Left | Right | Left | Right | |
| TH positive cells | 531 | 536 | 416 | 402 | 100 | 196 | 355 | 382 | 501 | 458 |
| TH/c-fos double positive cells | 209 | 106 | 112 | 12 | 52 | 51 | 191 | 153 | 104 | 48 |
| c-fos expression level (%) | 39.4 | 19.8 | 26.9 | 3.0 | 52.0 | 26.0 | 53.8 | 40.1 | 20.8 | 10.5 |
| P < 1e-5 | P < 1e-5 | P < 1e-5 | P < 1e-5 | P < 1e-5 | ||||||