Figure 1.

Anti-inflammatory effects of Hydrostatin-SN1 on LPS-induced MAPK pathway activity in BMDM cells. (A) Hydrostatin-SN1 inhibited LPS-induced MAPK and NF-κB pathway activation through the phosphorylation of JNK, ERK1/2, and p38. Relative protein levels were quantified using Quantity One software and expressed as optical density ratio. (B) Hydrostatin-SN1 decreased the phosphorylation of JNK. (C) Phosphorylation of ERK was decreased in Hydrostatin-SN1-treated cells. (D) Phosphorylation of p38 was decreased in Hydrostatin-SN1-treated cells. *P < 0.05, **P < 0.01 in Hydrostatin-SN1-treated cells vs. LPS-treated cells.