Table 2.
DNA methylation of defined t-PA and PAI-1 gene regions in ECT remitters and non-remitters.
| Baseline | Whole time course of ECT | ||||
|---|---|---|---|---|---|
| Region | Blood (n = 87) (R/NR (± SE), 95% CI) |
Blood (n = 28) (R/NR (± SE), 95% CI) |
PBMC (n = 21) (R/NR (± SE), 95% CI) |
NK cells (n = 21) (R/NR (± SE), 95% CI) |
T cells (n = 21) (R/NR (± SE), 95% CI) |
| Total t-PA | 0.455/0.472 (± 0.008/0.006) 0.440–0.470/0.460–0.483 |
0.479/0.459(± 0.005/0.006)** 0.469–0.488/0.447–0.471 |
0.647/0.622(± 0.008/0.009)* 0.632–0.663/0.605–0.640 |
0.615/0.592(± 0.009/0.010) 0.598–0.633/0.573–0.611 | 0.780/0.766(± 0.007/0.008) 0.766–0.794/0.751–0.781 |
| MHRE | 0.487/0.502 (± 0.008/0.006) 0.472–0.503/0.489–0.514 |
0.518/0.490(± 0.005/0.007)*** 0.508–0.527/0.477–0.503 |
0.690/0.654(± 0.008/0.009)** 0.675–0.706/0.637–0.671 |
0.655/0.621(± 0.009/0.010)* 0.636–0.673/0.601–0.641 |
0.804/0.781(± 0.007/0.008)* 0.790–0.817/0.766–0.796 |
| CREB | 0.381/0.407 (± 0.014/0.012) 0.353–0.409/0.384–0.430 |
0.375/0.393(± 0.009/0.012) 0.358–0.392/0.371–0.416 |
0.557/0.558(± 0.016/0.018) 0.526–0.589/0.521–0.594 |
0.559/0.573(± 0.014/0.015) 0.531–0.587/0.543–0.603 |
0.835/0.847(± 0.008/0.008) 0.819–0.851/0.830–0.864 |
| CREB + CTF/NF1 | 0.393/0.417 (± 0.012/0.010) 0.369–0.416/0.398–0.436 |
0.383/0.405(± 0.007/0.010) 0.370–0.424/0.370–0.397 |
0.553/0.568(± 0.013/0.015) 0.528–0.579/0.538–0.597 |
0.534/0.565(± 0.012/0.013) 0.512–0.557/0.540–0.590 |
0.801/0.824(± 0.008/0.009) 0.785–0.817/0.807–0.841 |
| Total PAI-1 | 0.156/0.142 (± 0.015/0.012) 0.127–0.186/0.118–0.165 |
0.164/0.152(± 0.009/0.013) 0.146–0.183/0.127–0.176 |
Measured in blood only | Measured in blood only | Measured in blood only |
The DNA methylation of defined t-PA gene regions differed between ECT remitters (R) and non-remitters (NR) in various sample types analyzed (i.e., the blood, the whole population of PBMCs (peripheral blood mononuclear cells), natural killer (NK) cells, and T cells). Results were calculated by mixed linear models (including Sidak’s correction) and are presented as mean [± standard error (SE)] and 95% confidence interval (CI). Total t-PA methylation includes three fragments (t-PA 2, t-PA 4, and t-PA 5), total PAI-1 methylation only one (PAI-1 2). For further information regarding the analyzed fragments, see Table S2 in the Supplements. MHRE, multi-hormone responsive enhancer, CREB, cAMP response element-binding protein, CTF/NF1, CCAAT box-binding transcription factor/nuclear factor 1. *p < 0.05, **p < 0.001, ***p < 0.0001.