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. 2020 May 4;10(12):5913–5921. doi: 10.1002/ece3.6327

FIGURE 2.

FIGURE 2

Immunocytochemical detection of pTAS2R20 at the cell surface. (a) The cell surface is labeled by a plasma membrane protein fused with GFP (mGFP). (b) The amino terminus of pTAS2R20 is tagged with FLAG (DYKDDDDK), and the FLAG epitope is detected by an anti‐FLAG primary antibody and an Alexa561‐conjugated secondary antibody. (c) Overlap of (a) and (b). Scale bar = 10 μm