Fig 2. C. elegans N2 and PB306 dauer animals do not respond to P. scaber washes with three different solvents.

(A) and (C) Plot of undiluted 1-octanol repulsive (R) and isoamyl alcohol diluted to 10⁻³ in ethanol attractive (A) reference control chemotactic indices for the N2 and PB306 dauers. (B) and (D) Plot of chemotactic indices of N2 and PB306 dauer C. elegans to undiluted P. scaber washes compared to neutral control references. Blue bars represent the neutral control of solvent alone. Yellow bars represent chemotactic indices to washes that were prepared from male isopods. Red bars represent chemotactic indices to washes that were prepared from female isopods. From left to right, each grouping of bars represents dimethyl sulfoxide (DMSO), deionized water, and ethanol washes (n = 40–50 animals per condition, per replicate). Error bars are standard deviation. No isopod wash was significantly different from its corresponding neutral control (see S5 Table for p values).