Fig 7. CD38⁺/CD14⁺/ABCA1⁺ (‘TP’ cells) myeloid cells are highly Mtb-infected.

A. The expression of CD38, CD14, and ABCA1 by non-PMN myeloid cells from the lungs of B6 mice, 3 wpi. Quadrants are defined as follows: Q1, CD38⁺CD14^-ve; Q2, CD38⁺CD14⁺; Q3, CD38^-veCD14⁺; and Q4, CD38^-veCD14^-ve. Each quadrant was then further divided into Abca1 negative and positive cells. This Boolean gating based on CD38, CD14, and ABCA1 expression defines 8 populations (labelled ‘A’ through ‘H’). B. The frequency of TP cells in each myeloid cell subsets. Each point represents one individual subject. C. CD38⁺CD14⁺ABCA1⁺ (‘TP’ cells) are enriched among YFP⁺ cells. Quadrants, based on CD38 and CD14 staining, were analyzed for YFP and ABCA1 expression. The highest frequency of YFP⁺ cells is found in CD38⁺CD14⁺ABCA1⁺ cells. D. For each of the gated cells populations labelled in Fig 7A as A through H, we determined: (1) the percentage of total myeloid cells in each gate; (2) the frequency of cells in each gate that are infected with Rv.YFP; (3) the contribution that each population makes to the total number of infected cells; and, (4) the MFI of the YFP⁺ cells in each gate. These data are representative of five independent experiments. Empty bars, ‘TN’ cells; red bars, ‘TP’ cells. Statistics, one-way ANOVA compared to ‘TN’ cells. Error bars, SD. *, p<0.05; **, <0.01, ***, <0.001, ****, <0.0001.