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. 2020 Jun 22;15:4441–4452. doi: 10.2147/IJN.S246797

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© 2020 Poier et al.

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Experimental design of repetitive cell exposure to ZnO NPs. HUVEC were exposed for 1 hr three subsequent times with washing steps and regeneration periods of 1 hr in between, followed by a 24-hr regeneration period. MTT assay was performed after 1 hr of treatment, after 3 hrs of treatment and after the 24-hr regeneration period. Comet assay was performed after 3 hrs of treatment.