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. 2020 Jun 22;15:4441–4452. doi: 10.2147/IJN.S246797

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© 2020 Poier et al.

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Dot blot. Dot blot indicated less VEGF release in HUVEC treated with ZnO NPs. Pictures of the negative control (A) and 5 µg/mL (B) show VEGF dots highlighted by red ellipses. Graph (C) shows the integrated density of the VEGF dots released by HUVEC after exposure to ECGM (c; negative control) and ZnO NPs (µg/mL) analyzed with ImageJ.