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. 2020 Jun 26;477(12):2401–2419. doi: 10.1042/BCJ20200368

Figure 7. Rv2827c–Rv2826c is a negatively autoregulating system in E. coli.

Figure 7.

(A) Promoter activity from upstream promoter regions of abiE (99 bp), and rv2827c–rv2826c and rv1044–rv1045 (500 bp for both) detected using lacZ transcriptional fusions. Both the abiE and rv2827c–rv2826c constructs are active, but the rv1044–rv1045 construct is not. Plotted data are normalized to the vector-only control. (B) Autoregulation of promoter activity by antitoxins. LacZ activity was measured from the abiE and rv2827c–rv2826c constructs with or without induction of the cognate antitoxin (AT, ±IPTG). Both AbiE and Rv2827c negatively autoregulate expression. Plotted data are normalized to the uninduced vector-only control. All data (A–B) are plotted as the means of triplicate data, and error bars show standard deviations from the mean.