Figure 3. Heterologous activity of Synechocystis and Arabidopsis cyclases in Rvi. gelatinosus.
Cyclase genes were tested in the Rvi. gelatinosus ΔbchEΔacsF RifR mutant by expression from a plasmid vector: (A) no plasmid, (B) pBB[cycI], (C) pBB[CHL27], (D) pBB[CHL27-YCF54], (E) pBB[acsF] and (F) pBB[cycI-ycf54]. Left, whole-cell absorbance spectra of the described strains, normalized and corrected for light scattering. Right, HPLC elution profiles of pigment extracts from the described strains standardized by cell number. The major peak in the traces was identified to be BChl a by the retention time and absorbance spectrum (inset).