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E. coli strains contained (A) IM-YCF54*, (B) IM-CHL27-ycf54, (C) IM-CRD1-ycf54, (D) IM-cycI-YCF54 and (E) IM-cycI-CGL78 plasmids. Pigments were extracted from the same number of cells. Elution of cyclase substrate and the product was monitored by fluorescence at 640 nm excited at 440 nm.
