Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
E. coli strains containing the indicated plasmids were grown, induced as for the in vivo cyclase assay and cells were harvested after 5 h induction. Clarified cell lysates were analyzed by SDS–PAGE with loading standardized by OD600, followed by transfer to a PVDF membrane for immunodetection. Cyclase subunits were detected by using antibodies raised against Arabidopsis CHL27, YCF54 and Synechocystis Ycf54.
