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. 2020 Jun 19;8(1):e000218. doi: 10.1136/jitc-2019-000218

Figure 1.

Figure 1

HD T cells become activated on CD3xCD19 dart treatment and are able to kill CD19+ cell-lines. (A–G) HD PBMCs were CTV labeled and left unstimulated or cultured in the presence of 100 ng/mL CD3xCD19 DART or anti-CD3/aCD28 antibodies. T cell proliferation (A) was measured after 4 days (n=4). (B–G) after 2 days induction of CD25 (B), degranulation measured by CD107a (C), IFNγ production (D), TNFα production (E), perforin production (F) and granzyme B production (G) were measured by fluorescence-activated cell sorting (FACS) (n=4–8). (H–I) FACS analysis of lysis of CTV labeled JeKo-1 cells cocultured in 1:1 (H) or 4:1 (I) E:T ratios and with different concentrations of the CD3xFITC and CD3xCD19 DART molecules for 24 hours and 4 days (n=2). *P<0.05; **P<0.01. CTV, cell trace violet; DART, dual affinity retargeting; HD, healthy donor; IFNγ, interferon γ; ns, not significant; PBMCs, peripheral blood mononuclear cells; TNFα, tumor necrosis factor-α.