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. 2020 Jun 19;8(1):e000218. doi: 10.1136/jitc-2019-000218

Figure 3.

Figure 3

CLL-derived T cells proliferate and activate and produce effector molecules on CD3xCD19 DART in exposure. (A–F) Age-matched HD* or CLL PBMCs were CTV labeled and left unstimulated or in the presence of 100 ng/mL CD3xCD19 DART or aCD3/aCD28 antibodies. T cell proliferation (A) was measured after 4 days (n=4). (B–G) After 2 days induction of CD25 (B), degranulation measured by CD107a (C), IFNy production (D), TNFα production (E), perforin production (F) and granzyme B production (G) were measured by FACS (n=4–8). *Data from HD PBMCs equal to data in figure 1A–G. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. CLL, chronic lymphocytic leukemia; CTV, cell trace violet; DART, dual affinity re-targeting; HD, healthy donor; IFNγ, interferon γ; PBMCs, peripheral blood mononuclear cells; TNFα, tumor necrosis factor-α.