Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | B6.SJL-Slc6a3tm1.1(cre)Bkmm/J; DATIRES-Cre | Bäckman et al., 2006 | JAX 006660, RRID:IMSR_JAX:006660 |
|
| Genetic reagent (M. musculus) | B6.129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze; Ai32 | Madisen et al., 2012 | JAX 012569, RRID:IMSR_JAX:012569 |
|
| Genetic reagent (M. musculus) | C57BL/6NTac-Syt1tm1a(EUCOMM)Wtsi/WtsiCnrm; Syt-1 floxed; Syt1lox/lox | Skarnes et al., 2011; Zhou et al., 2015; obtained from Dr. T.C. Südhof | EUCOMM (EM:06829), RRID:IMSR_EM:06829 |
Conditional Synaptotagmin-1 floxed mice |
| Cell line (H. sapiens) | HEK293T | ATCC | Cat#: CRL-3216, RRID:CVCL_0063 | |
| Recombinant DNA reagent | AAV-flex-oChIEF-citrine | Addgene; Lin et al., 2009 | Plasmid# 50973, RRID:Addgene_50973 |
|
| Chemical compound, drug | Nomifensine | Tocris | Cat. No. 1992 | |
| Chemical compound, drug | Tetrodotoxin | Tocris | Cat. No. 1078 | |
| Antibody | Mouse monoclonal IgG2a anti-Bassoon SAP7F407 (A85) | Enzo Life Sciences | Cat# ADI-VAM-PS003-F, RRID:AB_11181058 |
IHC (1:500) |
| Antibody | Guinea pig polyclonal anti-Tyrosine hydroxylase (A111) | Synaptic Systems | Cat# 213 104, RRID:AB_2619897 |
IHC (1:1000) |
| Antibody | Rabbit polyclonal anti-Synaptotagmin-1 antiserum (A24) | gift from Dr. T.C. Südhof | V216 | ICC (1:1000) |
| Other | Microdialysis probe | Harvard Apparatus | Item# CMA8309581 | |
| Other | Carbon fiber filaments | Goodfellow | Item# C 005722 |
|
| Software, algorithm | Fiji | Schindelin et al., 2012 | RRID:SCR_002285, https://imagej.net/Fiji/Downloads | Used for confocal synaptosome and 3D-SIM slice experiments |
| Software, algorithm | SoftWoRX | GE Healthcare | http://incelldownload.gehealthcare.com/bin/download_data/SoftWoRx/7.0.0/SoftWoRx.htm | Used for 3D reconstruction |
| Software, algorithm | Custom MATLAB code | Liu et al., 2018 | https://github.com/hmslcl/3D_SIM_analysis_HMS_Kaeser-lab_CL (Liu, 2017) | Used for analysis of 3D-SIM and synaptosome images |
| Software, algorithm | Prism8 | GraphPad | RRID:SCR_002798, https://www.graphpad.com/scientific-software/prism | Used for statistical analysis |