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. 2020 Jun 25;8(1):e000928. doi: 10.1136/jitc-2020-000928

Figure 2.

Figure 2

Ex vivo IFN-γ ELISpot responses. Vaccine immunogenicity was assessed by ex vivo IFN-γ ELISpot on freshly isolated peripheral blood mononuclear cells (PBMCs) stimulated with 8 pools of overlapping peptides covering the entire 5T4 protein sequence. Values of spot-forming cells (SFCs) per million PBMCs correspond to the sum of the responses to the single pools. (A) Dot plot representing peak responses for each individual patient compared with baseline (BL), pre-vaccination responses. Lines represent mean; the paired t-test p value is shown. (B) Comparison of peak responses in patients receiving homologous standard vaccination (MVA-MVA-MVA 5T4: MMM), heterologous standard vaccination (ChAdOx1-MVA-MVA 5T4: CMM) and heterologous accelerated vaccination regimes (ChAdOx1-MVA 5T4: CM), irrespective of cyclophosphamide (CTX) preconditioning. Lines represent mean; ns: not significant. (C) Comparison of peak responses between patients without CTX preconditioning (−CTX) or receiving CTX course (+CTX) for a week before each immunization, irrespective of vaccination regimen. Lines represent mean; ns: not significant. (D) Time-dependent dynamics of cellular responses to individual 5T4 peptide pools (p1 to p8) in 2 representative patients. Arrows represent time points of vaccination. AS, active surveillance; C, ChAdOx1.5T4; M, MVA.5T4; RP, radical prostatectomy.