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. 2020 Jun 25;8(1):e000928. doi: 10.1136/jitc-2020-000928

Figure 3.

Figure 3

Kinetics of 5T4 specific responses. Antigen-specific responses were measured before vaccination and at different time points post-vaccination by ex vivo IFN-γ ELISpot assay on freshly isolated peripheral blood mononuclear cells (PBMCs). Mean (±SEM) immune responses to 5T4 antigen over time are shown irrespective of cyclophosphamide preconditioning: (A) in the standard vaccination regimen arms; (B) in surgical patients receiving the accelerated vaccination regimen; (C) in active surveillance patients receiving the accelerated vaccination regimen. Arrows represent time points of vaccination. C/M: ChAdOx1.5T4 or MVA.5T4; C: ChAdOx1.5T4; M: MVA.5T4. (D) Freshly isolated PBMCs from several patients were cultured for 2 weeks in the presence of low-dose hIL-2 and the total 5T4 peptide pool. IFN-γ ELISpot assay was performed on cultured cells and baseline responses were compared with responses measured at weeks 16 to 24.