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. 2020 Jun 24;8(1):e000704. doi: 10.1136/jitc-2020-000704

Figure 1.

Figure 1

Linkage of ovalbumin (OVA) antigens to extra domain A (EDA) improves immunological synapse. Dendritic cell (DC) incubated with equimolar amounts of SIINFEKL, EDA-SIINFEKL, EDA-OVA, OVA or left untreated, were co-cultured with purified CD8+ T cell from OT-I mice. Then cells were fixed, resin embedded and thin sectioned to be visualized by electron microscopy. (A) Representative micrographs from each group (upper row) and details of contacting areas or synapses (lower row). Magnification bars in upper row correspond to 0.5 µm, and in lower row correspond to 200 nm. (B) Analyzes of longest close apposition (nm), the fraction of the synapses showing one close apposition >400 nm and the mean size of continuous region of close apposition corresponding to 10–12 conjugates/group, measured in electron micrographs from thin sections of cells. Results are representative of two independent experiments. *P<0.05, **p<0.01.