Figure 5.
Therapeutic efficacy of subcutaneous (s.c.) mEDA-HPVE7-16 immunotherapy combined with cisplatin chemotherapy. C57BL/6 mice were challenged s.c. with 1×105 TC-1 P3 (A15) tumor cells and treated as indicated (n=8 per group). mEDA-HPVE7-16 (3 nmol), mEDA (3 nmol), Poly IC (50 µg/mouse) and peptide E743–77 (20 µg/mouse) were s.c. administered at days 5, 8 and 11, whereas cisplatin (5 mg/kg body weight) was administered intraperitoneally (i.p.) at days 5 and 8. (A) Tumor size (average of two perpendicular diameters (mm)) was measured at regular intervals. The ratio of tumor-free mice/total mice is shown at day 90 for each treatment. (B) A Kaplan-Meier plot is depicted for each treatment. Mice were sacrificed when tumor diameters reached 18 mm or based on the health of the individual animals. PBS, phosphate-buffered saline.