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. Author manuscript; available in PMC: 2021 Jun 25.
Published in final edited form as: Chem Commun (Camb). 2020 Jun 25;56(51):6977–6980. doi: 10.1039/d0cc02612c

Figure 3.

Figure 3.

(a) Viability of MCF-7 cells treated with LysoCR for 2 h and irradiated with 808 nm laser (5 W/cm2, 10 min). (b) Observation of lysosomal membrane permeabilization of MCF-7 cells that had been co-incubated with 10 μM LysoSQ and 100 μM LysoCR for 2 h, irradiated with 808 nm laser (5 W/cm2, 10 min) and then treated with Hoechst 33342. The LysoSQ emits deep-red fluorescence (ex. 655/40 nm, em. 716/40 nm) and the Hoechst 33342 emits blue fluorescence. (c) Effect of laser irradiation (808 nm, 5 W/cm2, 10 min) on MCF-7 cells that had been incubated sequentially with 100 μM LysoCR for 2 h, followed by 1 μg/mL AO for 10 min. The AO generates red fluorescence (ex. 562/40 nm, em. 624/40 nm) in acidic lysosomes and emits green fluorescence (ex. 450/90 nm, em. 500/50 nm) at near neutral pH. Scale bar = 20 μm. Cross-sectional analysis along the yellow line in the representative cell images reflects spatial change in red (red curve) and green (blue curve) fluorescence intensity.