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. Author manuscript; available in PMC: 2020 Dec 17.
Published in final edited form as: Nature. 2020 Jun 17;582(7813):566–570. doi: 10.1038/s41586-020-2396-4

Extended Data Fig. 7. 7α-dehydroxylation of CDCA in vivo.

Extended Data Fig. 7.

Combined extracted ion chromatograms showing the conversion of CDCA to LCA by a C. sporogenes strain harboring the complete bai operon on three plasmids (MF001) versus a control strain of C. sporogenes harboring the transporter baiG (MF012). The strains were cultivated with 1 μM CA for 72 hr; an acetone extract of the culture supernatant was analyzed by HPLC-MS. The single asterisk indicates isoLCA, and the double asterisk is provisionally assigned as isoCDCA. This experiment was performed once.