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. Author manuscript; available in PMC: 2021 Jul 1.

Published in final edited form as: Neurogastroenterol Motil. 2020 Mar 13;32(7):e13838. doi: 10.1111/nmo.13838

Figure 6. — LTA induces oxidative stress in myenteric neurons. A-B, Like LPS, LTA increased the amount of DHE staining per ganglionic area (A) and in the entire field of view (B) after 12 hours. C, Sample images showing nNOS (green) and DHE (red) staining in duodenal myenteric ganglia (outlined in overlay) of control, LPS and LTA treated samples. D-E, Like LPS, LTA increased the anti-glutathione synthase (GS) immunoreactivity (D) and increased the GS positive cells per ganglionic area (E). F, Sample images showing nNOS neurons (green) and anti-glutathione synthase (red) immunoreactivities in duodenal myenteric ganglia of control, LPS and LTA treated (12 hours) muscularis.

Figure 6. — LTA induces oxidative stress in myenteric neurons. A-B, Like LPS, LTA increased the amount of DHE staining per ganglionic area (A) and in the entire field of view (B) after 12 hours. C, Sample images showing nNOS (green) and DHE (red) staining in duodenal myenteric ganglia (outlined in overlay) of control, LPS and LTA treated samples. D-E, Like LPS, LTA increased the anti-glutathione synthase (GS) immunoreactivity (D) and increased the GS positive cells per ganglionic area (E). F, Sample images showing nNOS neurons (green) and anti-glutathione synthase (red) immunoreactivities in duodenal myenteric ganglia of control, LPS and LTA treated (12 hours) muscularis.