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. 2020 Jun 26;11:3236. doi: 10.1038/s41467-020-17052-4

Fig. 4. Interaction between SLFN12 and PDE3A is required for nauclefine-induced cell death.

Fig. 4

a, b The PDE3 inhibitors cilostazol (1 µM) or trequinsin (50 nM) were co-treated with nauclefine (200 nM) in HeLa cells with Flag-SLFN12 stably expressed. ATP levels were measured to assess cell viability a (n = 4, examined in three independent experiments). This is a representative result (mean ± SD) and the p values from Student’s two-tailed unpaired t-tests are indicated. *p < 0.05; **p < 0.01; ***p < 0.001; ns not significant. Cells were treated as in a for 20 h, followed by immunoprecipitation (b). Cilo denotes cilostazol, and Treq denotes trequinsin. c Flag-SLFN12 was transfected into HeLa cells stably expressed WT or mutant PDE3A. Nauclefine (200 nM) was then administered and cells were treated for 20 h, followed by immunoprecipitation. d The indicated cells were treated by nauclefine (200 nM) for 36 h, followed by assessment of cell viability based on cellular ATP levels (n = 3, examined in two independent experiments). The western blots are representative of results from two independent experiments.