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. 2020 Jun 26;10:10425. doi: 10.1038/s41598-020-66829-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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POE immunoassay probe selectivity to for the antisense strands of four unique siRNAs in a mixture. All siRNA molecules were added to a single calibration curve from 0.3 to 16,700 fM in Sprague Dawley serum. Independent probe sets were used to detect their corresponding antisense strands. Theoretical and back-calculated results were graphed in GraphPad Prism. Non-parametric Friedman test was performed using GraphPad Prism and showed no statistically significant difference at 5% significance level (p value = 0.983) demonstrating no changes in calibration curves when siRNAs prepared as a mixture. Mean data is shown with error bars representing standard deviation.