Table 1.
Accuracy and precision results within-day and between-days for the qualification of HPRT.siRNA antisense POE immunoassay in Sprague-Dawley serum and liver tissue homogenate.
Qualification parameters of HPRT-siRNA POE immunoassay in Sprague-Dawley serum (antisense strand) | Qualification parameters of HPRT-siRNA POE immunoassay in Sprague-Dawley liver homogenate (antisense strand) | ||||||||
---|---|---|---|---|---|---|---|---|---|
Within-day (n = 18) | Between-day (n = 18) | Within-day (n = 18) | Between-day (n = 18) | ||||||
%CV | %Bias | %CV | %Bias | %CV | %Bias | %CV | %Bias | ||
LQC | 7.0 to 13.8 | −20.2 to 4.0 | 16.1 | −5.8 | LQC | 3.0 to 19.6 | −8.2 to 8.4 | 11.6 | −2.1 |
MQC | 1.0 to 19.3 | −11.5 to 1.3 | 18.7 | −4.6 | MQC | 6.0 to 32.8 | −11.3 to 16.9 | 16.4 | 0.9 |
HQC | 4.1 to 24.2 | −18.4 to 24.8 | 25.7 | 0 | HQC | 12.0 to 29.6 | −34.4 to 24.0 | 26.7 | −3.2 |