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. 2020 Jun 26;10:10490. doi: 10.1038/s41598-020-66966-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bronchioles become smaller in their cross-sectional area and diameter with age. Representative 40x images of H&E-stained distal lung bronchioles from (a) 3 month old and (b) 22 month old mice. Scale bars are 150 µm. Dotplots depicting (c) Circularity of the whole bronchiole and the lumen, (d) Area of the whole bronchiole, the bronchiolar lumen and the bronchiolar wall, (e) Thickness of the bronchiolar wall, (f) Diameter of the whole bronchiole and bronchiolar lumen. Circles represent 3 month old mice, and squares represent 22 month old mice. >7 images were analysed per mouse. Error bars are standard deviations. P-values refer to two-tailed T-test results.