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. 2020 Jun 26;6:27. doi: 10.1038/s41523-020-0169-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Two serially sectioned 6 μm sections from formalin-fixed paraffin-embedded tissue blocks are generated. One slide is stained with hematoxylin and eosin and scanned for annotation. After the slide is annotated by the pathologists, the areas of interest are mapped on the unstained slide. After enzyme digestion and matrix application, the mass spectra of individual annotated areas are obtained.