Table 2.
Regression equations, coefficient of determinations (R2), and p-values of each response of crude polysaccharides from KUM-DB using UAE (KUM-U).
| Responses | Regression equation | Adj. R2 | p-value |
|---|---|---|---|
| Yield (%) | −8.31 + 0.33 X1 + 0.16 X2 + 0.14 X3 − 1.84 × 10−3X1X2 − 1.02 × 10−3 X1X3 − 1.80 × 10−3 X2X3 − 4.04 × 10−3X12 − 2.43 × 10−4X22 − 1.64 × 10−4X32 | 0.905 | 0.0036 |
| IC50 DPPH value (mg/mL) | 5.97 − 0.21 X1 − 0.08 X2 + 0.08 X3 + 2.75 × 10−4X1X2 − 1.00 × 10−4 X1X3 + 1.06 × 10−3 X2X3 − 4.99 × 10−3 X12 + 3.79 × 10−4X22 + 1.44 × 10−3X32 | 0.909 | 0.0033 |
| IC50 ABTS value (mg/mL) | 12.9 − 0.73 X1 − 0.15 X2 + 6.07 × 10−3X3 + 2.61 × 10−3 X1X2 + 3.90 × 10−4X1X3 + 4.71 × 10−5X2X3 + 0.01 X12 + 1.03 × 10−3 X22 + 4.32 × 10−4X32 | 0.889 | 0.0052 |
|
FRAP value (µM Fe2+/g) |
140.3 + 1.11 X1 + 2.16 X2 − 0.53 X3 − 1.00 × 10−2X1X2 + 0.01 × 1X3 −0.01 X2X3 − 0.02 X12 − 0.01 X22 + 6.25 × 10−3X32 |
0.914 | 0.0029 |
p-value indicates a significant difference at 95% confidence (p ≤ 0.05). X1 = ratio of KUM-DB to water (w/v), X2 = extraction temperature (°C), X3 = extraction time (min).